Determination of seasonal changes of spider mite (Acari: Tetranychidae) den- sities and species composition on kudzu vine and soybean (Fabaceae) in Japan with the use of phosphoglucomutase zymograms*

Identification of spider mites based on morphological characteristics is difficult because morphological differences between species may be subtle and in some groups, including the Tetranychus Dufour species, detectable only in adult males, which usually correspond to a small proportion of a population. The utility of an alternative method, phosphoglucomutase (PGM) zymogram, is demonstrated in this study. Using this method, we were able to discriminate females of each of 13 known Japanese Tetranychus species. We examined the species composition and seasonal density changes on kudzu vine [Pueraria montana var. lobata (Willd.) Sanjappa and Pradeep], a fabaceous weed, between 1997 and 1999, and on soybean [Glycine max (L.)] between 1999 and 2001. On kudzu vine, spider mite populations showed two types of seasonal fluctuation, one characterized by a single peak in September or October and the other by two peaks, in June and September. Five spider mite species were found on this plant species, with T. pueraricola Ehara & Gotoh being dominant throughout the 3-year period and accounting for 75.6–96.9% of all females. On soybean, spider mite populations showed three types of seasonal fluctuation, characterized by one peak (August), two peaks (August, November), and three peaks (June, August, October), respectively. On this plant, eight species were found, T. pueraricola being dominant in 1999 (54.7%), T. parakanzawai Ehara in 2000 (72.6%) and T. kanzawai Kishida in 2001 (69.2%). Such annual variation in dominance was probably determined by the order of invasion of soybean fields.